Using next generation sequencing technologies to assess the diet of the Mediterranean Shag (Phalacrocorax Aristotelis) and implication of these technologies for high - throughput study and monitoring of marine biodiversity.

Kasapidis, Panagiotis; Boyer, F.; Christidis, A.; Kristoffersen, J.B.; Oulas, A.; Nikolioudakis, N.; Fric, J.

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dc.contributor.author	Kasapidis, Panagiotis	en
dc.contributor.author	Boyer, F.
dc.contributor.author	Christidis, A.
dc.contributor.author	Kristoffersen, J.B.
dc.contributor.author	Oulas, A.
dc.contributor.author	Nikolioudakis, N.
dc.contributor.author	Fric, J.
dc.date.accessioned	2016-03-11T11:48:50Z
dc.date.issued	2016-03-11
dc.identifier.uri	http://hdl.handle.net/123456789/497
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.subject	Diet analysis	en
dc.subject	Marine biodiversity	en
dc.subject	Next generation sequencing	en
dc.subject	DNA metabarcoding	en
dc.subject	Mediterranean Shag	en
dc.subject	Phalacrocorax Aristotelis	en
dc.subject	NGS	en
dc.title	Using next generation sequencing technologies to assess the diet of the Mediterranean Shag (Phalacrocorax Aristotelis) and implication of these technologies for high - throughput study and monitoring of marine biodiversity.	en
heal.type	conferenceItem
heal.generalDescription	Oral Presentation.	en
heal.classification	Diet	en
heal.classification	Biology	en
heal.classificationURI	N/A-Diet
heal.classificationURI	N/A-Biology
heal.keywordURI	N/A-Diet analysis
heal.keywordURI	N/A-Marine biodiversity
heal.keywordURI	N/A-Next generation sequencing
heal.keywordURI	N/A-DNA metabarcoding
heal.keywordURI	N/A-Mediterranean Shag
heal.keywordURI	N/A-Phalacrocorax Aristotelis
heal.keywordURI	N/A-NGS
heal.identifier.secondary	GB/7013
heal.dateAvailable	2016-03-11T11:53:50Z
heal.language	en
heal.access	free
heal.recordProvider	HCMR	en
heal.recordProvider	ΕΛΚΕΘΕ	el
heal.publicationDate	2013-10
heal.bibliographicCitation	Kasapidis P., Boyer F., Christidis A., Kristoffersen J.B., Oulas A., Nikolioudakis N., Fric J. (2013). Using next generation sequencing technologies to assess the diet of the Mediterranean Shag (Phalacrocorax Aristotelis) and implication of these technologies for high - throughput study and monitoring of marine biodiversity. In: MARBIGEN Conference 2013 : Mediterranean marine biodiversity in view of climate change and the invasion of alien species. Heraklion Chamber, Crete, Greece, 7- 9 October, 2013.Heraklion: HCMR/IMBBC. pp.37 .	en
heal.abstract	Since 2005, next generation sequencing (NGS) technologies have revolutionized the study of genomes, as well as the study of bacterial diversity. More recently, these technologies are increasingly used to study the biodiversity of various groups of eukaryotes at different environments, by genetically analysing bulk environmental samples, an approach termed 'DNA metabarcoding'. This approach was used to assess the diet of a seabird, the Mediterranean shag, within the frame of the EU LIFE project "ConShagAudMIBAGR" using both conventional prey identification and NGS. Regurgitated pellets containing undigested prey remains were collected from different colonies of the species in Greece. First, conventional prey analysis was carried out on the pellets, by identifying morphologically the indigestible part remains, mainly fish otoliths. Then, the same pellets were subjected to amplicon sequencing using the 454 GS-FLX platform. In order to check how well molecular approaches perform in comparison to conventional ones for prey identification at the species' level, we analysed the same samples by both techniques. The genetic analysis produced results that were generally concordant with the otolith analysis (although there were some discrepancies) and seems to be reliable and cost effective method. DNA metabarcoding can be also applied in the study and monitoring of marine biodiversity, especially for plankton and benthic communities, which is currently based on the morphological identification of species and has many limitations. HCMR is about to implement this approach in this field with the establishment of marine observatories, and the advantages and limitations of this methodology are discussed.	en
heal.sponsor	the Seventh Framework Programme (FP7-REGPOT-2010-1)	en
heal.publisher	HCMR/IMBBC	en
heal.publisher	ΕΛΚΕΘΕ/ΙΘΑΒΒΥΚ	el
heal.fullTextAvailability	true
heal.conferenceName	MARBIGEN Conference 2013 : Mediterranean marine biodiversity in view of climate change and the invasion of alien species.	en
heal.conferenceItemType	abstract
heal.coverage.spatial	Greece	el